Plasmid

Part:BBa_K3779027

Designed by: Junfeng Hui   Group: iGEM21_NWU-CHINA-B   (2021-09-27)


pPIC9K-SS-bgly

The strain combined with the target gene


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1110
    Illegal EcoRI site found at 1460
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 13310
    Illegal EcoRI site found at 18557
    Illegal EcoRI site found at 27987
    Illegal XbaI site found at 279
    Illegal XbaI site found at 441
    Illegal XbaI site found at 720
    Illegal XbaI site found at 14120
    Illegal XbaI site found at 19367
    Illegal XbaI site found at 28797
    Illegal PstI site found at 1914
    Illegal PstI site found at 3155
    Illegal PstI site found at 5710
    Illegal PstI site found at 7537
    Illegal PstI site found at 8677
    Illegal PstI site found at 11232
    Illegal PstI site found at 13059
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1110
    Illegal EcoRI site found at 1460
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 13310
    Illegal EcoRI site found at 18557
    Illegal EcoRI site found at 27987
    Illegal NheI site found at 8359
    Illegal NheI site found at 13881
    Illegal NheI site found at 14828
    Illegal NheI site found at 19128
    Illegal NheI site found at 20075
    Illegal NheI site found at 28558
    Illegal NheI site found at 29505
    Illegal PstI site found at 1914
    Illegal PstI site found at 3155
    Illegal PstI site found at 5710
    Illegal PstI site found at 7537
    Illegal PstI site found at 8677
    Illegal PstI site found at 11232
    Illegal PstI site found at 13059
    Illegal NotI site found at 7800
    Illegal NotI site found at 13322
    Illegal NotI site found at 18569
    Illegal NotI site found at 27999
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1110
    Illegal EcoRI site found at 1460
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 13310
    Illegal EcoRI site found at 18557
    Illegal EcoRI site found at 27987
    Illegal BglII site found at 4164
    Illegal BglII site found at 6567
    Illegal BglII site found at 9686
    Illegal BglII site found at 12089
    Illegal BglII site found at 17336
    Illegal BglII site found at 24209
    Illegal BglII site found at 26766
    Illegal BamHI site found at 7504
    Illegal BamHI site found at 13026
    Illegal BamHI site found at 18273
    Illegal BamHI site found at 27703
    Illegal XhoI site found at 1743
    Illegal XhoI site found at 2999
    Illegal XhoI site found at 7758
    Illegal XhoI site found at 13280
    Illegal XhoI site found at 18527
    Illegal XhoI site found at 23044
    Illegal XhoI site found at 27957
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1110
    Illegal EcoRI site found at 1460
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 13310
    Illegal EcoRI site found at 18557
    Illegal EcoRI site found at 27987
    Illegal XbaI site found at 279
    Illegal XbaI site found at 441
    Illegal XbaI site found at 720
    Illegal XbaI site found at 14120
    Illegal XbaI site found at 19367
    Illegal XbaI site found at 28797
    Illegal PstI site found at 1914
    Illegal PstI site found at 3155
    Illegal PstI site found at 5710
    Illegal PstI site found at 7537
    Illegal PstI site found at 8677
    Illegal PstI site found at 11232
    Illegal PstI site found at 13059
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1110
    Illegal EcoRI site found at 1460
    Illegal EcoRI site found at 7788
    Illegal EcoRI site found at 13310
    Illegal EcoRI site found at 18557
    Illegal EcoRI site found at 27987
    Illegal XbaI site found at 279
    Illegal XbaI site found at 441
    Illegal XbaI site found at 720
    Illegal XbaI site found at 14120
    Illegal XbaI site found at 19367
    Illegal XbaI site found at 28797
    Illegal PstI site found at 1914
    Illegal PstI site found at 3155
    Illegal PstI site found at 5710
    Illegal PstI site found at 7537
    Illegal PstI site found at 8677
    Illegal PstI site found at 11232
    Illegal PstI site found at 13059
    Illegal AgeI site found at 7866
    Illegal AgeI site found at 13388
    Illegal AgeI site found at 18635
    Illegal AgeI site found at 28065
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None